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Joao Pimentel PDF Print E-mail

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Background

B.Sc. and M.Sc. in Electrical Engineering (University of Brasília, Brazil)

Ph.D. in Electrotechnology and Materials (Czech Technical University in Prague, Czech Republic)

 

Project description

The focus of the research project was to develop on-chip elements for infrared and optical analysis of suspended cells. Infrared spectroscopy can be used for cancer investigation, e.g. by distinguishing between healthy skin and melanoma. With the detection of single cells in suspension, melanoma cells can therefore be detected in lymph fluid without the need of labels.

The research dealt with the miniaturization of optofluidic elements (e.g. on-chip lenses) to improve a system's detection capability, minimize sample volumes needed for analysis, and reduce diagnosis time. Two main approaches were taken:

1. A separation-and-recirculation system that continuously ressuplies two liquids in a microfluidic device, enabling for example the operation of optofluidic lenses without input of liquids.
2. Microfluidic structures prepared by a new and simple fabrication process. They consisted of fully sealed parylene channels and chambers embedded in a silicon wafer. The ease in preparing functional microfluidic structures out of these materials is very promising for realizing devices dedicated to IR analysis of cells in suspension.

 

Network meetings

Basel, Switzerland, March 18-21, 2014

Copenhagen, Denmark, August 25-28, 2014

Bremen, Germany, February 23-24, 2015

 

Courses

Courses from the network meeting in Basel, March 2014

Courses from the network meeting in Copenhagen, August 2014

 

Publications in conference proceedings

S. van den Driesche, J.V. Pimentel, D. Puchberger-Enengl, L. Brandhoff, M.J. Vellekoop, Easy-to-realise Polyvinylsiloxane Microfluidic Connectors for PDMS Chips, Procedia Eng. 120 (2015), 675-678. doi:10.1016/j.proeng.2015.08.720

E. Tolstosheeva, J.V. Pimentel, A. Schander, L. Kempen, M.J. Vellekoop, W. Lang, Fabrication of parylene channels embedded in silicon using a single parylene deposition step, Proc. SPIE 9518 (2015), 951811. doi: 10.1117/12.2179780

 
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